Clickmer Systems

What are Clickmers®?

Synthetic affinity reagents known as Clickmers are chemically modified ssDNA oligonucleotides that, based on their sequence and modifications, form three-dimensional structures that adaptively bind to their target molecules with high affinity and specificity.

The introduction of side chains/modifications through the Nobel Prize-winning ‘Click’ chemistry provides increased chemical variability and flexibility compared to standard aptamers, which increases the probability of developing an excellent target binder.

What are the benefits of employing Clickmers in your technological platforms?

  1. Chemical synthetic synthesis means improved batch-to-batch continuity

  2. Improved turn-around time – Target-binding Clickmers can be developed within a few weeks with subsequent characterisation bespoke to the client’s requirements

  3. Ethical development – no animal testing required

  4. Flexible assay read-out: enzymatic, fluorescent or even RT-qPCR-based according to the client’s needs

  5. Increased binding surface of the target protein landscape in comparison to custom/commercial antibodies raised against short peptides

A Nobel Prize Winning Technology - ‘Click’ Chemistry

APIS Assay Technologies have the knowledge and capability to develop Clickmer products using this Nobel Prize winning technology.


Clickmers Webinar

Title: Optimising Bioprocessing with Clickmers: Improving Flexibility and Efficiency in Purification

Looking for an affinity reagent for bioprocessing that adapts to your procedure and not the other way round?

Webinar Highlights: 

• Uncover the fundamentals of Clickmers and their potential in bioprocessing  

• Learn how Clickmers can be specifically developed for the needs of your production process   

• Learn about the fast development time, high stability and versatility in recognising target structures from proteins to small molecules

Custom Clickmer Development

We develop Clickmers for use as research tools, diagnostics, or therapeutics - designed to integrate seamlessly into your platform.

If you're interested in a custom Clickmer for your novel target, or would like to evaluate one of our pre-developed candidates, get in touch. A member of our technical team will be happy to discuss your project.

  • Clickmers provide next-generation antibody-analogue tools that enable researchers and diagnostic developers to overcome the limitations of antibody-based technology and batch-to-batch variability

  • Our Clickmer Systems Development Service offers a structured milestone-defined development pipeline focused on understanding client requirements and project aims

Clickmer Products

If you would like to discuss a custom development on your novel target or try one of our pre-developed Clickmers below then please contact us and one of the technical team can discuss the project with you.

For more information on all our available Clickmer reagents and Clickmer-based immunoassay kits click here.

How are Clickmers selected?

Our proprietary Click-SELEX is an in vitro evolutionary process of iterative cycles for the selection of Clickmers, which are consequently isolated and enriched through PCR to generate a pool of specific target-binding Clickmers (see below).

A major benefit of the SELEX process is the ability to adapt the selection process to meet client requirements, e.g. by selecting a Clickmer in the presence of different mediums, including serum.

Click-SELEX is an in vitro evolutionary process of iterative cycles

Featured Clickmer Application - Protein Purification

Ideal reagents for sample preparation and affinity protein purification

Clickmers high binding specificity in complex matrices reduces the number of purification and refinement steps, and improves yield of highly pure protein for use in downstream applications.

As Clickmers do not bind to misfolded and denatured proteins, only native-like functional protein is purified. Furthermore, high quality of the purified protein is maintained by our proprietary, customised Clickmer selection process for conditional binding so that the protein can be eluted under non-denaturing conditions, which also simplifies column regeneration.

Thanks to the ease of covalent conjugation to resin such as Sepharose and the batch-to-batch continuity, Clickmers provide the means to purify your protein of choice reproducibly, at high quality.

APIS Clickmers

Clickmer development is available to integrate into our client’s platform or to integrate as part of our Contract Assay Development service.

For more information on this product please contact us.