APIS DPYD HT qPCR Kit
The APIS DPYD HT qPCR Kit is designed for fast and efficient detection of seven DPYD alleles. Each reaction identifies both wildtype (WT) and variant alleles, directly from blood or extracted genomic DNA (gDNA).
The kit’s rapid turnaround time, cost-effectiveness, and easy integration into existing qPCR workflows, makes it a reliable and accessible option for a variety of research applications.
Variants detected by the APIS DPYD HT qPCR Kit
The APIS DPYD HT qPCR Kit enables the qualitative detection of specific DPYD gene variants using a high throughput, multiplexed real-time PCR format. The kit provides a scalable and efficient solution for detecting these variants in various research settings.
Table 1. DPYD variants detected by the APIS DPYD HT qPCR Kit
APIS DPYD qPCR Kit shows excellent concordance with alternative research genotyping methods
gDNA extracted from 29 specimens with known DPYD variant status (characterised via NGS, microarrays or qPCR), Seraseq DPYD DNA Mutation Mix (SeraCare, Cat. No.0750-9502) and one synthetic DNA sample for each variant were assessed with the APIS DPYD HT qPCR Kit.
Table 2: Concordance of the APIS DPYD HT qPCR Kit with other research genotyping methods
*One of which was the Seraseq DNA mutation mix, *One synthetic DNA per variant tested
APIS DPYD qPCR Kit Specifications
The kit has undergone testing as part of APIS unique RUO assay development pipeline, including both in silico and wet laboratory testing. As a result, researchers can depend on the kit for consistent and high-quality detection of DPYD variants. A positive and negative control (PC and NTC), which monitor assay set up and assay performance, are also supplied with the kit.
Ordering Information
| Assay Name | Test Type | Kit Size | Catalogue Number | Price |
|---|---|---|---|---|
| APIS DPYD HT qPCR Kit | RUO | 24 samples plus controls | 04201 | Available upon request |
To order the APIS DPYD HT qPCR Kit or to learn more about how our assay can elevate your research capabilities, please contact us below.
