APIS Precision
Expression Assays
APIS Precision Expression Assays are a versatile solution for the accurate and precise quantitative expression analysis of a wide range of important human biomarkers. The assays aim to help researchers to better understand critical cellular processes and ultimately develop more effective treatments or diagnostic methods.
APIS Assay’s Precision Expression Assays
Gene-specific forward and reverse primers, as well as a fluorescently labelled probe. Probes are supplied as standard labelled with FAM on the 5’ end and a quencher on the 3’ end. Custom probe configuration is also available upon request
Standard kit size is 100 reactions which is customisable upon request
Assays are optimised for use in one-step RT-qPCR reactions
Assays are made to order and undergo stringent QC testing before release and shipment
The APIS Precision Expression Assays feature target specific primers and probes that have been optimised for performance.
Each assay has undergone testing as part of our comprehensive assay development pipeline, which includes in silico and wet laboratory testing. This extensive testing ensures consistent assay performance across various sample types, instruments, and reagents.
APIS Precision Expression Assay Specifications
| Condition | Specification |
|---|---|
| Format | Tube, primer-probe |
| PCR method | RT-qPCR (either one or two step) |
| Instrument | Any qPCR instrument with compatible cycling and emission detection capabilities |
| Probe modifications | 5’ FAM; 3’ BHQ1 |
| No. of reactions | 100 or customisable |
| Concentration | 20X |
| Shipping conditions | Room temperature |
| Recommended One-Step RT-qPCR master mixes | Thermo Fisher Scientific™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit • Integrated DNA Technologies™ PrimeTime™ One-Step RT-qPCR Master Mix • New England Biolabs® Luna® Universal One-Step RT-qPCR Kit • QIAGEN ZipScript One-Step RT-qPCR Kit |
Benefits of Using APIS Precision Expression Assays
High specificity - assays have undergone extensive in silico analysis and wet-lab testing
High efficiency - assays have >90% PCR efficiency guaranteed
Easy to use - Our assays are designed to be user-friendly, with a simple protocol that involves adding cDNA or RNA to the reaction. There is no requirement for melt curve analysis post-run, saving you time and effort.
Reagent flexibility - Our assays have been tested with a variety of commercially available PCR master mixes, providing you with greater flexibility in your experimental design
Ordering Information
We currently have several assays available in the table below, and additional assays will be available for commercial use soon.
| Assay Name | Assay Type | Gene Synonyms | Kit Size (reactions) | Product Code |
|---|---|---|---|---|
| GAS5 | Non-coding RNA Assay | NCRNA00030, SNHG2 | 100 | 01301 |
| TAp63-regulated lncRNA 1 | Non-coding RNA Assay | TROLL-1, ENSG00000236947 | 100 | 01401 |
| TAp63-regulated lncRNA 3 | Non-coding RNA Assay | TROLL-3, ENSG00000231889 | 100 | 01501 |
| TAp63-regulated lncRNA 7 | Non-coding RNA Assay | TROLL-7, ENSG00000251562 | 100 | 01601 |
| TAp63-regulated lncRNA 4 | Non-coding RNA Assay | TROLL-4, ENSG00000272168 | 100 | 01701 |
| TAp63-regulated lncRNA 6 | Non-coding RNA Assay | TROLL-6, ENSG00000266651 | 100 | 01801 |
| TAp63-regulated lncRNA 8 | Non-coding RNA Assay | TROLL-8, ENSG00000258471 | 100 | 01901 |
| TAp63-regulated lncRNA 9 | Non-coding RNA Assay | TROLL-9, ENSG00000237976 | 100 | 02001 |
| ROR1 | Gene Expression Assay | NTRKR1 | 100 | 00601 |
| ROR2 | Gene Expression Assay | NTRKR2, BDB, BDB1 | 100 | 00701 |
| TROP2 | Gene Expression Assay | GA733-1, EGP-1 | 100 | 00801 |
| PD-L1 | Gene Expression Assay | B7-H, B7H1, PDL1, B7-H1, PDCD1LG1 | 100 | 00901 |
| ERBB1 | Gene Expression Assay | ERRP, EGFR, HER1 | 100 | 01001 |
| ERBB2 | Gene Expression Assay | NGL, NEU, HER-2, CD340, HER2, c-ERB2, c-ERB-2, MLN-19, p185(erbB2) | 100 | 01101 |
| ERBB3 | Gene Expression Assay | HER3, LCCS2 | 100 | 01201 |
