APIS Breast Cancer Subtyping Kit
The APIS Breast Cancer Subtyping Kit is a highly reproducible, RT-qPCR-based kit for detecting mRNA expression of standard breast cancer biomarkers (HER2, ER, PR, Ki67) and novel proliferative biomarkers from core-needle biopsy (CNB) or resected formalin-fixed paraffin embedded (FFPE) breast tumour tissue. Receptor tyrosine-protein kinase erbB-2 (HER2), oestrogen receptor (ER), progesterone receptor (PR), and marker of proliferation (Ki67) biomarkers play a key role in breast cancer.
The kit also includes a novel four-gene proliferative signature that comprises four markers (MKI67, CCNA2, KIF23, and PCNA) associated with proliferation. These markers are expressed throughout all cell cycle stages, providing a more precise representation of tumour proliferation.
The APIS Breast Cancer Subtyping Kit delivers precise results for standard biomarkers and a novel proliferative signature in less than 5 hours when processing 10 samples.
The APIS Breast Cancer Subtyping Kit contains an internal control (IC), a synthetic RNA sequence, which monitors for assay set up, reagent performance and interfering substances. Positive and Negative Controls (PC and NTC), which monitor run validity and reagent performance are also supplied with the kit.
A Comprehensive Molecular Profile for Breast Cancer
The APIS Breast Cancer Subtyping Kit uses One Step RT-qPCR with dye-linked oligonucleotides to detect sequence amplification. To selectively amplify the mRNA sequence, at least one of the oligonucleotides used to generate the amplicon is spanning an exon-exon boundary. The probe binds to the target sequence between the primers.
APIS Breast Cancer Subtyping Kit Performance
Comparison of APIS Breast Cancer Subtyping Kit expression measure (ΔCt) with IHC% nuclei staining for each target. A strong correlation between IHC% staining and the APIS Breast Cancer Subtyping Kit can be observed.
Y axis; APIS RNA gene expression (ΔCt value) with assays cut-off (dashed line) indicating positive/negative calling. Cut off values valid only when analysis performed using QS5™Dx instrument. X axis; IHC positive nuclei (%).
APIS Breast Cancer Subtyping Kit
The APIS Breast Cancer Subtyping Kit is intended for Research Use Only (RUO). Not for use in diagnostic procedures.
For more information on this product please contact us.
Ordering Information
| Product Name | Test Type | Kit Size | Catalogue Number | Price |
|---|---|---|---|---|
| APIS Breast Cancer Subtyping Kit | RUO | 24 samples in duplicate plus controls | 00402 (distributed by Biocartis) | Available upon request |
| APIS Breast Cancer Subtyping Kit | RUO | 24 samples in duplicate plus controls | 00403 (distributed by APIS) | Available upon request |
To order the APIS Breast Cancer Subtyping Kit or to learn more about how our assay can elevate your breast cancer research capabilities, please contact us below.
Biocartis currently distributes to the following countries: Austria, Belgium, Denmark, France, Germany, Greece, Hungary, Italy, Liechtenstein, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of Cyprus, Republic of Ireland, Slovenia, Spain, Sweden, Switzerland. APIS is the distributor for all other countries.