Clickmer Systems
What are Clickmers®?
Synthetic affinity reagents known as Clickmers are chemically modified ssDNA oligonucleotides that, based on their sequence and modifications, form three-dimensional structures that adaptively bind to their target molecules with high affinity and specificity.
The introduction of side chains/modifications through the Nobel Prize-winning ‘Click’ chemistry provides increased chemical variability and flexibility compared to standard aptamers, which increases the probability of developing an excellent target binder.
What are the benefits of employing Clickmers in your technological platforms?
Chemical synthetic synthesis means improved batch-to-batch continuity
Improved turn-around time – Target-binding Clickmers can be developed within a few weeks with subsequent characterisation bespoke to the client’s requirements
Ethical development – no animal testing required
Flexible assay read-out: enzymatic, fluorescent or even RT-qPCR-based according to the client’s needs
Increased binding surface of the target protein landscape in comparison to custom/commercial antibodies raised against short peptides
A Nobel Prize Winning Technology - ‘Click’ Chemistry
APIS Assay Technologies have the knowledge and capability to develop Clickmer products using this Nobel Prize winning technology.
How are Clickmers selected?
Our proprietary Click-SELEX is an in vitro evolutionary process of iterative cycles for the selection of Clickmers, which are consequently isolated and enriched through PCR to generate a pool of specific target-binding Clickmers (see below).
A major benefit of the SELEX process is the ability to adapt the selection process to meet client requirements, e.g. by selecting a Clickmer in the presence of different mediums, including serum.
Click-SELEX is an in vitro evolutionary process of iterative cycles
Custom Clickmer Development
Clickmer development is available to integrate into our client’s platform, or to integrate as part of our Contract Assay Development service
Clickmers provide next-generation antibody-analogue tools that enable researchers and diagnostic developers to overcome the limitations of antibody-based technology and batch-to-batch variability
Our Clickmer Systems Development Service offers a structured milestone-defined development pipeline focused on understanding client requirements and project aims
